螢光光譜/成像

MicroTime100 螢光成像系統

MicroTime100 螢光成像系統

正置型时间分辨荧光显微镜,增强型,含LDH-D-C-405、LDH-D-C-485、PDL 828-S、2-D高分辨率压电扫描、2个SPAD探测器、标准物镜及100x物镜(NA 1.4)、PicoHarp 300、SPT-1+2、电脑、显示器以及运输、包装、安装。

產品說明

MicroTime100 螢光成像系統是時間分辨共軛焦顯微系統規格之一,時間分辨共軛焦顯微系統包括擁有皮秒瞬時分辨率和超分辨成像功能的單分子級別顯微系統,同時,還有可與主流廠商(Olympus,Nikon,Leica,Zeiss)生產的LSM顯微鏡搭配的螢光升級系統,以實現時間分辨的相關實驗應用。

產品特色

  • 整合雷射光源,正置顯微鏡鏡身和探測元件的完整共軛焦系統
  • 375nm ~ 810nm波長範圍二極體雷射
  • 最多可支持4獨立探測通道
  • 可用於2D生命週期成像的XY壓電式掃描台
  • 可選釐米級別大範圍掃描台

產品應用

  • 時間解析螢光研究
  • 單分子光譜
  • 時間解析光致螢光光譜研究(TRPL)
  • 時間解析光致螢光光譜成像(PLIM)
  • 脈沖交錯勵磁(PIE)
  • 螢光共振能量轉移(FRET)
  • 模式匹配分析法(針對FLIM)
  • 螢光相關光譜(FCS)
  • 螢光相關光譜(FLCS)
  • 反集束探測

產品參數

Sub-System Specification
Excitation system
  • Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz inside a compact fibre coupling unit
  • Wavelengths between 375 and 900 nm
  • Single or multichannel laser driver
  • New: 560 nm picosecond pulsed excitation with the LDH-D-TA-560
Microscope
  • Upright microscope BX43 from Olympus
  • Side ports and back port still accessible (for e.g. widefield imaging or TIRF)
  • Transmission illumination unit included
  • Manual positioning stage
Objectives
  • Air objectives with 20x and 40x magnification (standard)
  • Various high end objectives available (Oil / Water immersion, air spaced, IR/UV-enhanced, TIRF or long working distance objectives upon request
Scanning
  • Optional 2-dimensional piezo scanning table with 80 µm x 80 μm scan range at nominal 1 nm positioning accuracy, mounted in objective scanning fashion
  • Optional: large area scanning table with centimeter range
Main optical unit
  • Confocal detection setup in a compact housing with up to four parallel detection channels for PMA and PMA Hybrid detectors, two detection channels for SPAD detectors
  • Prealigned exchangeable major dichroic mirror holder
  • All optical elements easily accessible and adjustable
Detectors
  • Single Photon Avalanche Diodes
  • Hybrid-Photomultiplier Tubes
  • Photomultiplier Tubes
Data acquisition
  • Based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time Resolved (TTTR) measurement mode
  • Simultaneous data acquisition of up to two channels
Software
  • SymPhoTime 64 for imaging and FCS applications
  • FluoFit software for lifeime measurement applications without scanning