螢光光譜/成像

MicroTime200 螢光成像系統

MicroTime200 螢光成像系統

倒置型时间分辨荧光显微镜,增强型,含LDH-D-C-440、 LDH-D-C-485、LDH-D-C-640、PDL 828-L、自由空间接口、3-D压电扫描及3-D PiFoc、2个Hybrid-PMA探测器、2个SPAD探测器,出光口、标准物镜及60x水浸物镜(NA 1.2)、HydraHarp 400、 SPT 64 1+2、电脑和2个显示器,以及运输、包装、安装。

產品說明

MicroTime200 螢光成像系統是時間分辨共軛焦顯微系統規格之一,時間分辨共軛焦顯微系統包括擁有皮秒瞬時分辨率和超分辨成像功能的單分子級別顯微系統,同時,還有可與主流廠商(Olympus,Nikon,Leica,Zeiss)生產的LSM顯微鏡搭配的螢光升級系統,以實現時間分辨的相關實驗應用。

產品特色

  • 整合雷射光源,倒置顯微鏡鏡身和多探測頻道的完整共軛焦系統
  • 375nm ~ 900nm波長範圍二極體雷射
  • 最多可支持4獨立探測頻道
  • 時間相關單光子計數(TCSPC)模式和TTTR模式可選
  • 可用於2D或3D成像的壓電式掃描
  • 兩個可選的出光接口,可以選裝例如光譜儀等設備
  • 專業化,全功能,圖像化配套軟體SymPhoTime64
  • 可選雙聚焦FCS,同時AFM/FLIM,紫外測量
  • 可選安裝最新高解析螢光顯微技術(STED)升級

產品應用

  • 時間解析螢光研究
  • 單分子光譜
  • 時間解析光致螢光光譜研究(TRPL)
  • 時間解析光致螢光光譜成像(PLIM)
  • 脈沖交錯勵磁(PIE)
  • 螢光共振能量轉移(FRET)
  • 模式匹配分析法(針對FLIM)
  • 螢光相關光譜(FCS)
  • 螢光生命週期相關光譜(FLCS)
  • 雙聚焦螢光相關性光譜(2fFCS)
  • 螢光各向異性研究
  • 反集束探測

產品參數

Excitation System
  • Picosecond diode lasers with adjustable output power and repetition rates up to 80 MHz inside a compact fibre coupling unit
  • Wavelengths between 375 and 900 nm
  • Single or multi-channel laser driver
  • Optional: external laser (e.g. Titanium:Sapphire laser)
Microscope
  • Inverted microscope IX 71 or IX 81 from Olympus
  • Side ports and back port still accessible (for e.g. widefield imaging or TIRF)
  • Transmission illumination unit included
  • Special manual positioning stage with 25 mm range
  • Sample stage for 20 x 20 mm cover slips or microscope slides
  • Optional: Cryostat for low temperature measurements
  • Optional: Combination with Atomic Force Microscope (AFM)
Objectives
  • Air objectives with 20x and 40x magnification (standard)
  • Various high end objectives available (Oil / Water immersion, air spaced, IR/UV-enhanced, TIRF or long working distance objectives)
Scanning
  • Computer controlled
  • 2-dimensional piezo scanning table with 80 x 80 μm scan range at nominal 1 nm positioning accuracy, other scanning ranges are possible
  • PIFOC for 3-dimensional imaging, 80 μm range at nominal 1 nm positioning accuracy
  • Optional: objective scanning with 80 x 80 μm scan range at nominal 1 nm positioning accuracy
  • Optional: large area scanning table with centimetre range
Main optical unit
  • Confocal detection setup in a compact housing with up to four parallel detection channels
  • Prealigned exchangeable major dichroic mirror holder
  • All optical elements easily accessible and adjustable
  • CCD camera and photodiode for beam diagnostics
  • Variable beam splitting units
  • Exit ports to connect external devices
Detectors
  • Single Photon Avalanche Diodes, Photomultiplier Tubes or Hybrid-Photomultiplier Tubes (available soon)
Data acquisition
  • Based on the method of Time-Correlated Single Photon Counting (TCSPC) in the unique Time-Tagged Time Resolved (TTTR) mode
  • Simultaneous data acquisition of up to four channels
System software
  • Easy-to-use and comprehensive Windows system and analysis software
  • 1-, 2- and 3-dimensional data acquisition based on the versatile TTTR file format
  • Data archiving in workspace, time-gating for all methods, separation of up to four detector signals, data export features
  • Point measurement analysis: MCS trace, FCS / FLCS calculation and fitting, TCSPC histogram and fitting, on/off-state histogram, burst size analysis, FRET histogram incl. Pulsed-Interleaved Excitation, Photon counting histogram, Lifetime histogram
  • Imaging measurement analysis: fluorescence intensity images, fluorescence lifetime images, time-gated analysis, TCSPC histogram for region of interest
  • Scripting language (“STUPSLANG”) for user-defined analysis procedures